Screening of differentially expressed genes in placentas with hepatitis b virus infection by suppression subtractive hybridization technique 乙型肝炎病毒感染胎盤組織差異表達(dá)基因的篩選與確定
Dominating methods in wheat gene cloning include homology - based cloning , map - based cloning , suppression subtractive hybridization , ddrt - pcr etc 目前,小麥基因的克隆方法主要有同源克隆、圖位克隆、差減雜交、差異顯示等。
Among these techniques , the suppression subtractive hybridization ( ssh ) method has been shown to be quick and highly efficient , and it generates less false - positive clones 將實驗方的雙鏈cdna分成兩組,分別與兩種不同的接頭連接( adaptor1andadaptor2r ) 。
In virtue of the technique of suppression subtractive hybridization , we had successfully constructed a subtractive library of d . involucrata sprouting bract by taking homochronous leaf as driver in this study 本研究利用新近發(fā)展的抑制性差減雜交技術(shù)( ssh )成功構(gòu)建了珙桐幼嫩苞片和葉片差減文庫。
In previous study , janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization , and registered in genbank 在前期的研究工作中,本室姜宏等通過建立小鼠隱睪模型,運用抑制消減雜交法( suppressionsubtractivehybridization , ssh )克隆出24個小鼠睪丸生精細(xì)胞凋亡相關(guān)基因的est ,并在genbank中登錄。
After the screening , the expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l . under salt stress had been done . methods : construction and screening of the subtractive library : to construct a cdna subtractive library of aloe vera l . under salt stress , the leaves of aloe vera l were removed from the seedlings which were treated with 300 mm nacl . suppression subtractive hybridization ( ssh ) was carried out and a subtractive library was constructed 鹽脅迫下庫拉索蘆薈葉子中nadp -蘋果酸酶基因的表達(dá)鑒定:為了檢測蘆薈鹽脅迫下庫拉索蘆薈cdna消減又庫的構(gòu)建篩選及nadp一蘋果酸酶基因的鹽誘導(dǎo)表達(dá)中nadp一蘋果酸酶基因‘刀叻心)的表達(dá)和nadp一蘋果酸酶( nadp一ma1ieenzyme )蛋白的積累是否受著高鹽的誘導(dǎo),我們利用耐鹽品種庫拉索蘆薈( a了口。
Part 1 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of subtractive cdna library of mitten crab ( eriocheir japonica sinensis ) ovary two subtracted cdna libraries of mitten crab ( eriocheir japonica sinensis ) ovaries at two successive developmental stages were constructed by suppression subtractive hybridization ( ssh ) 第一部分中華絨螯蟹卵巢發(fā)育相關(guān)基因的篩選1中華絨螯蟹卵巢差減cdna文庫的構(gòu)建應(yīng)用抑制性差減雜交技術(shù),構(gòu)建了中華絨螯蟹卵巢兩個發(fā)育時期的差減cdna文庫。
A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ) . the cdna subtractive library after amplification included 100 blue clones and 400 white clones , 75 ones of which were selected to prepare for plasmid . identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector 構(gòu)建高消減效率的+ gz重復(fù)暴露大鼠腦cdna消減文庫,擴增后cdna文庫包含約400個白色克隆和100個藍(lán)色克隆,克隆飽滿清晰,隨機挑取75個白色克隆,制備質(zhì)粒后,以ecor酶切分析,表明大部分克隆入質(zhì)粒載體。
In this study , sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group . the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury 本研究利用大鼠重復(fù)+ 10gz暴露引發(fā)腦損傷的動物模型,觀察腦的病理學(xué)改變;應(yīng)用抑制性消減雜交技術(shù)篩選+ gz重復(fù)暴露大鼠腦的差異表達(dá)基因,旨在初步探討+ gz重復(fù)暴露致腦損傷的分子機制。
Furthermore , suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes , and forward suppression subtractive cdna library of cold - regulated genes was constructed . the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver . subtractive cdna library was differentially screened through cdna macroarray , six hundreds and four cdna clones were identified as cold specifically induced or highly expressed ( 5 )應(yīng)用抑制差減雜交( suppressionsubtractivehybridization , ssh )方法,構(gòu)建冷誘導(dǎo)表達(dá)的正向抑制差減cdna文庫,低溫處理的幼苗為tester ,常溫處理為driver ,通過cdna微陣列差異篩選cdna文庫,得到604個低溫誘導(dǎo)或表達(dá)增強的候選克隆,對其中的84克隆進(jìn)行dna測序,去除冗余的cdna ,在genbank中進(jìn)行核酸和蛋白質(zhì)同源性的比較和功能分析,共有36個單一序列,其中12個cdna在genbank數(shù)據(jù)庫沒有同源的序列。
百科解釋
Subtractive hybridization is a technology that allows for PCR-based amplification of only cDNA fragments that differ between a control (driver) and experimental transcriptome. Differences in relative abundance of transcripts are highlighted, as are genetic differences between species.